AP Biology 12 - Unit 4: Molecular Genetics and Biotechnology

Learning Goal: Are we controlled by our DNA?
- Concept 1: Analyzing the regulation of gene expression and virus cycles (Ch 18, 19 and Inv 7 Part 3)
- Concept 2: Analyzing and utilizing biotechnology tools (Ch 20, AP Inv 8, and AP Inv 9)
- Concept 3: Analyzing genomes and their evolution (Ch 21, AP Inv 3)

Date
What are we Learning?
Jan 6
Introduction
- Current Research: How to grow a retina!
- Learning Intentions for Unit
- Jigsaw activity for: The Molecular basis of Inheritance (DNA structure/function/replicatio) and From Gene to Protein (protein synthesis)

Concept 1: Analyzing the regulation of gene expression and virus cycles (Ch 18, 19 and Inv 7 Part 3)
- Discussion with ppt

- Complete Mastering Biology online mini-assignment "Gene Expression in Prokaryotes"
- Optional Mastering Biology online mini-assignments "Review of DNA Replication" and "Protein Synthesis"
Jan 8 (x)
Concept 1: Analyzing the regulation of gene expression and virus cycles (Ch 18, 19 and Inv 7 Part 3)
- Discussion with ppt continued
- Trp operon animation
- Lac operon animation
- Act Out!
- Handout: Gene Expression in Bacteria
- KEY


- Complete Mastering Biology online mini-assignment "Gene Expression in Eukaryotes"
Jan 12
Concept 1: Analyzing the regulation of gene expression and virus cycles (Ch 18, 19 and Inv 7 Part 3)
- Remember Histones?: Video - DNA packing
- Animation: Regulated Transcription
- Challenge:
Can you explain this concept to someone else (not taking biology)?  Importance, process...
- Read p. 356-363 in Campbell, using p. 132 in Holtzclaw as a guide
- Handout: Gene Expression in Eukaryotes
- KEY
- 2009 #4 Free Response
Jan 14
Concept 1: Analyzing the regulation of gene expression and virus cycles (Ch 18, 19 and Inv 7 Part 3)
- Act Out!
- Discuss Gene Expression in Eukaryotes

- Read/skim p. 366-378 in Campbell and p. 132-134 in Holtzclaw, focusing on embryonic development and cancer.
- Answer Questions #1, 3, 5, 6, 7, 8, 11, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 30, 33 from p. 143-147 in Holtzclaw.
 
Jan 19
Concept 1: Analyzing the regulation of gene expression and virus cycles (Ch 18, 19 and Inv 7 Part 3)
- Chat about Interesting Book: The Immortal Life of Henrietta Lacks - by Rebecca Skoot 

- Virus Discussion with ppt
- Handout: Viruses, Viroids, and Prions
- KEY
Jan 21
Checkpoint #1 - Concept 1

Concept 2: Analyzing and utilizing biotechnology tools (Ch 20, AP Inv 8, and AP Inv 9)
- Discussion with ppt
- Intro to PCR and Gel Electrophoresis
- Read 396-416
- Handout and KEY
Jan 23
Concept 2: Analyzing and utilizing biotechnology tools (Ch 20, AP Inv 8, and AP Inv 9)
Bring a Device
Virtual Lab AP Investigation 9: Biotechnology - Restriction Enzyme Analysis of DNA 
- Activity: Virtual PCR Lab
- Activity: Virtual Electrophoresis Lab
- Activity: LabBench Gel Electrophoresis
 - Investigation from Campbell: How can gel electrophoresis be used to Analyze DNA? Handout and KEY

- Complete Mastering Biology online mini-assignment "Biotechnology"
Jan 27
Concept 2: Analyzing and utilizing biotechnology tools (Ch 20, AP Inv 8, and AP Inv 9)
- Discuss with ppt and animations
- Bacterial Transformation: Prep for AP Investigation 8
Jan 29
Bl 1-1
Concept 2: Analyzing and utilizing biotechnology tools (Ch 20 and AP Inv 8)
- Lab Inv 8 - Molecular Genetics (Transformation - Let's make bacteria glow!): Day 1 - Set Up Transformation
Jan 30 LUNCH
(1:00)
Concept 2: Analyzing and utilizing biotechnology tools (Ch 20 and AP Inv 8)
- Lab Inv 8 - Molecular Genetics (Transformation - Let's make bacteria glow!) Day 2 - Results

Picture
Thank you to Gagarin for the photo :)  
Successfully transformed E coli from 2012

Awesome info about GFP (green fluorescent protein)
- Click here, here, here, and here
Other transgenic organisms using GFP
- Click here and here
Medical Research application
- Click here and here
Feb 2
Concept 2: Analyzing and utilizing biotechnology tools (Ch 20, AP Inv 8, and AP Inv 9)
- Discuss Transformation Results. Ensure you are through with your explanations.
- Short Free Response Flashback to Biochem/Cells Unit
- Long Free Response Surprise! 2007#4

- Complete Mastering Biology online mini-assignment "Genomes and their Evolution"
Feb 4
Concept 3: Analyzing genomes and their evolution (Ch 21, AP Inv 3): retrotransposons, evo-devo, homeoboxes
Bring a Device
- Respect to Sanger
- AP Investigation 3: Comparing DNA Sequences to understand Evolutionary Relationships with BLAST
- Read: Making an Embryo
- Read: DNA double take
- Watch: RNA Interference
- Read: Transposons
Feb 10
AP Lab Investigation 8 DUE
Checkpoint #2 - Concept 2 and 3
- Article Link: DNA's New Alphabet 

- Science Article: "Creation of a Bacterial Cell Controlled by a Chemically Synthesized Genome": Read (skim) and highlight all terminology you recognize!  Pay special attention to the introduction, the figures, and the discussion.  
For next class, be ready to discuss: What is the relevance/significance of this research article?
Feb 12
Review
- Free Response Examples (2014 #2a, 5, 8, 2013 #5, 2012 #3, 2009 #4, 2009B #1, 2007 #4, 2002 #1)
- All Questions in Holtzclaw for Topic 5 (Molecular Genetics), Inv 8 (Bacterial Transformation), Inv 9 (Rest Enz Analysis of DNA)

- AP Test Format: Read pg. 10-24 in Holtzclaw
- Our Unit Test: 75 min: (40 min: 28 MC, 2 Grid-in; 3 min: Free Response Reading; 32 min: 1 long free, 2 short free)
 
Feb 16
Unit Test